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目的比较苏州、南京两地女性宫颈上皮细胞人乳头瘤病毒(HPV)感染基因分布情况。方法采用基因扩增结合基因芯片技术对3084例宫颈上皮细胞标本进行23种HPV基因型别检测。结果 3084例女性宫颈上皮细胞标本,HPV总阳性率为21.37%(659/3084),苏州、南京地区HPV总阳性率分别为35.32%(481/1362)、10.34%(178/1722),一重感染阳性率分别为24.08%(328/1362)、8.89%(153/1722),多重感染阳性率分别为11.23%(153/1362)、1.45%(25/1722),两地HPV总阳性率及一重、多重感染阳性率比较差异有统计学意义(P<0.05)。结论基因扩增结合基因芯片检测技术可应用于宫颈细胞标本HPV检测,对女性宫颈HPV感染分子流行病学调查研究具有重要意义。
Objective To compare the gene distribution of human papillomavirus (HPV) infection in female cervical epithelial cells in Suzhou and Nanjing. Methods Totally 2384 HPV genotypes were detected in 3084 cervical epithelial samples by gene amplification and gene chip technology. Results The total positive rate of HPV in 3084 cases of cervical epithelial cells was 21.37% (659/3084). The positive rates of HPV in Suzhou and Nanjing were 35.32% (481/1362) and 10.34% (178/1722), respectively, The positive rates of multiple infections were 11.23% (153/1362) and 1.45% (25/1722) respectively, the positive rates of HPV were 24.08% (328/1362) and 8.89% (153/1722) , The positive rate of multiple infection was significantly different (P <0.05). Conclusion Gene amplification combined with gene chip detection technology can be applied to HPV testing of cervical cell samples, and is of great significance to investigate the molecular epidemiology of cervical HPV infection in women.