Owing to its great medicinal and oamental values,Dendrobium officinale is frequently adulterated with other Dendrobium species on the market.Unfortunately,the utilization of the common DNA markers ITS,ITS2,and matK+rbcL is unable to distinguish D.officinale from 5 closely related species of it (D.tosaense,D.shixingense,D.flexicaule,D.scoriarum and D.aduncum).Here,we compared 63 Dendrobium plastomes comprising 40 newly sequenced plastomes of the 6 species and 23 previously published plastomes.The plastomes of D.officinale and its closely related species were shown to have conserved genome Structure and gene content.Comparative analyses revealed that small single copy region contained higher variation than large single copy and inverted repeat regions,which was mainly attributed to the loss/retention of ndh genes.Furthermore,the intraspecific sequence variability among different Dendrobium species was shown to be diversified,which necessitates a cautious evaluation of genetic markers specific for different Dendrobium species.By evaluating the maximum likelihood trees inferred from different datasets,we found that the complete plastome sequence dataset had the highest discriminatory power for D.officinale and its closely related species,indicating that complete plastome sequences can be used to accurately authenticate Dendrobium species.