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目的研究20(S)-原人参二醇皂苷元对肝星状细胞凋亡及激活的作用。方法利用体外培养的HSC细胞,通过台盼蓝染色、MTT法、Hoechst33258细胞核染色、DNA Ladder以及Western Blot和荧光定量PCR等方法检测细胞凋亡,利用肝纤维化大鼠模型研究肝纤维化及HSC细胞激活,同时利用TAA诱导的肝纤维化模型研究PPD对肝纤维化抑制作用和HSC细胞激活的影响。结果 PPD可诱导HSC细胞凋亡同时也抑制肝星状细胞的激活。结论 PPD具有较明显的抗肝纤维化的作用,可作为肝纤维化治疗新的的潜在药物。
Objective To study the effect of 20 (S) -ginsenol-diol sapogenin on apoptosis and activation of hepatic stellate cells. Methods HSC cells cultured in vitro were used to detect cell apoptosis by trypan blue staining, MTT assay, Hoechst33258 cell nuclear staining, DNA Ladder, Western Blot and real-time PCR. Liver fibrosis and HSC Cell activation, and TAA-induced liver fibrosis model was used to study the effect of PPD on hepatic fibrosis and HSC cell activation. Results PPD could induce HSC cell apoptosis as well as inhibit HSC activation. Conclusions PPD has a more obvious anti-hepatic fibrosis effect and can be used as a potential new drug for the treatment of liver fibrosis.