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为鉴别我国山丘、平原疫区杜氏利什曼原虫分离株，采用内切酶AluⅠ对其kDNA进行酶切。结果显示实验各株均有500bp片段；L．d．汶川人株、L．d．四川人株、L．d．甘肃人株（GS2）、L．d．四川犬株、L．infantum及L．d．Jeddah具有210bp片段；L．d．四川人株、Ld．甘肃人株（GS2）及L．d．四川犬株具有120bp片段；L．d．汶川人株、L．d．甘肃人株（GS2）和L．d．四川犬株具有400bp和280bp片段。L．d．甘肃犬株也具有400bp片段；L．infantum和L．d．Jeddah具有280bp片段。提示山丘疫区不同分离株的kDNAAluⅠ酶切片段相近，其中人株与犬株的相似；山丘疫区分离株与平原疫区分离株的酶切片段之间有较大差异，山丘疫区分离株与L．infantum的酶切片段之间既有相似又有差异。
In order to identify the Leishmania donovani isolates in the hills and plains of China, the kDNA was digested with AluI. The results showed that each experiment has 500bp fragments; L. d. Wenchuan human strain, L. d. Sichuan strain, L. d. Gansu strain (GS2), L. d. Sichuan dog strains, L. infantum and L. d. Jeddah has a 210 bp fragment; d. Sichuan strain, Ld. Gansu strain (GS2) and L. d. Sichuan canine strains with 120bp fragment; d. Wenchuan human strain, L. d. Gansu strain (GS2) and L. d. Sichuan canine strains have 400 bp and 280 bp fragments. L. d. Gansu canine strains also have a 400bp fragment; infantum and L. d. Jeddah has a 280 bp fragment. Suggesting that kDNAAluI digested fragments of different isolates from the endemic areas were similar, in which the human strains were similar to the canine strains. There were significant differences between the segmented isolates from the plague endemic areas and the isolates from the plague endemic areas. Isolate and L. There are similarities and differences between infantum fragments.