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在大鼠妊娠的第4天,宫内一次性注入醋酸棉酚0.5mg(0.1ml),4～11天时实验组每天注射孕酮5mg,对照组注射等量生理盐水,第12天处死动物剖检。结果,孕酮能有效地对抗醋酸棉酚的抗着床作用。在切除卵巢延缓着床模型的大鼠,相应实验组宫内一次注入抗生育剂量棉酚,对照组注入等量溶剂,结果表明外源性雌激素不能逆转宫内用醋酸棉酚的抗着床作用。实验组子宫液用SDS-聚丙烯酰胺凝胶电泳分析发现宫内注入醋酸棉酚可使孕酮诱导的分子量7万以下的蛋白带减少,但对雌二醇诱导的分子量大于9万的蛋白带无作用,这可能是其抗着床作用的机理之一。
On the 4th day of pregnancy, intrauterine injection of gossypol acetate 0.5mg (0.1ml) was performed at one time. During 4 to 11 days, progesterone 5mg was injected daily in the experimental group. The control group was injected with equal volume of normal saline and the animals were sacrificed on the 12th day. Inspection. As a result, progesterone is effective against the anti-implantation effect of gossypol acetic acid. Rats in the ovariectomy-delayed implantation model were given an intrauterine injection of an antifertility dose of gossypol in the corresponding experimental group and an equal amount of solvent in the control group. The results showed that exogenous estrogen could not reverse the anti-implantation of intrauterine gossypol acetate. effect. The uterine fluid of the experimental group was analyzed by SDS-polyacrylamide gel electrophoresis and it was found that intrauterine injection of gossypol acetate can reduce the progesterone-induced protein bands with a molecular weight of less than 70,000, but the estradiol-induced protein bands with a molecular weight greater than 90,000. No effect, this may be one of the mechanisms of its anti-implantation effect.