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在发现１８种氨基酸特别是色氨酸在ＴＯＹＯＰＥＡＲＬＨＷ－４０Ｓ树脂所填色谱柱上表现出非理想排阻行为的基础上，以色氨酸在ＨＷ柱上的保留时间为指标，探讨不同的流动相改良剂对ＨＷ凝胶与色氨酸之间相互作用的影响，从而阐明这种反常行为是疏水、离子交换以及氢键三者综合作用的结果，而疏水作用是最主要的影响因素。实验结果说明流动相组成对ＨＷ凝胶色谱行为有明显的影响作用，这不但有助于确立ＨＷ凝胶理想的排阻色谱条件，而且为利用其所具有的吸附性能分离分析肽类和氨基酸等小分子物质奠定了基础。
Based on the finding that 18 kinds of amino acids, especially tryptophan, exhibited non-ideal exclusion behavior on TOYOPEARLHW-40S resin column, the retention time of tryptophan on HW column was used as an index to investigate the influence of different mobile phases The effect of the modifier on the interaction between HW gel and tryptophan indicated that this abnormal behavior was the result of the combination of hydrophobicity, ion exchange and hydrogen bonding. The hydrophobic effect was the most important factor. The experimental results show that the mobile phase composition has a significant effect on the HW gel chromatography behavior, which not only helps establish the ideal exclusion chromatography conditions for the HW gel, but also separates and analyzes the peptides and amino acids by using the adsorption properties Small molecule material laid the foundation.