本文提出了采用内标法 ,将 HPLC用于吡罗昔康凝胶有效成分测定的新方法。讨论了流动相及内标的选择 ,进样量和空白基质的影响等。在优化的实验条件下 ,吡罗昔康浓度在 0 .0 2～ 0 .2 mg/m L范围内 ,其浓度与吡罗昔康和内标的峰面积之比呈线性关系。应用于实际样品分析 ,其结果与紫外分光光度法一致 ,平均回收率为 99.6% ,相对标准偏差为 1 .0 %。方法简单 ,准确度高 ,专属性强 ,可用于吡罗昔康凝胶样品的稳定性考察和质量控制 In this paper, a new method for the determination of active ingredients of piroxicam gel using internal standard method was proposed. The choice of mobile phase and internal standard, the effect of injection volume and blank matrix, etc. were discussed. Under optimized experimental conditions, the concentration of piroxicam was in the range of 0.2-2.2 mg / m L, and its concentration was linear with the ratio of the area of ​​piroxicam to the internal standard. Applied to the actual sample analysis, the results are consistent with UV spectrophotometry, the average recovery was 99.6%, the relative standard deviation of 1.0%. The method is simple, accurate and specific, and can be used for the stability inspection and quality control of piroxicam gel samples