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铁对人体的健康十分重要,缺铁已对人类健康和世界经济造成巨大影响。针对我国水稻生物强化育种工作中存在的样品加工过程铁污染严重,以及缺少适合育种群体大规模简便、快速的铁浓度检测手段,本研究发展了一种振荡研磨加工方法和基于邻二氮菲染色的比色测定方法。在研磨程度相同的情况下,经铁制精米机械加工的18个样品中有一半样品的铁浓度显著高于振荡研磨加工的,表明铁制精米加工机械对水稻精米加工存在明显的铁污染。本研究发明的精米振荡研磨加工方法,可以消除加工机械对样品加工过程中的铁污染。尽管对59个铁生物强化后代的测定平均铁浓度邻二氮菲比色法要比ICP-MS高出2.98mgkg-1,但两者的相关系数高达0.87,表明该测定方法适用于大批量育种群体精米铁浓度的初步筛选。与ICP-MS相比,邻二氮菲比色法具有简便、快速和低成本的特点。以铁浓度高的水稻种质为供体,导入广西本地高产品种背景,对分离后代采用上述加工和检测方法进行筛选,育成新品系精米的铁浓度比原品种提高了3倍。
Iron is very important to human health, and iron deficiency has had a huge impact on human health and the world economy. In view of the serious iron pollution in the process of rice bio-breeding and the lack of large-scale simple and rapid iron concentration detection methods suitable for the breeding population, an oscillating grinding method and a method based on o-phenanthroline staining Colorimetric determination method. In the same degree of grinding, the iron concentration of half of the 18 samples machined from iron milled rice was significantly higher than that of the vibration milled rice, indicating that iron milled rice has obvious iron pollution to rice milled rice. The grinding method of the polished rice oscillation invented in this study can eliminate the iron pollution of the processing machinery during the sample processing. Although the average ferrous phenanthrene concentration of 59 iron-fortified offspring was 2.98 mg kg-1 higher than that of ICP-MS, the correlation coefficient was as high as 0.87, indicating that this method is suitable for large-scale breeding population Preliminary screening of milled rice iron concentration. Compared with ICP-MS, phenanthroline colorimetric method is simple, fast and low-cost characteristics. Taking the rice germplasm with high iron concentration as the donor, we imported the background of the local high-yielding cultivars in Guangxi and screened the offspring using the above-mentioned processing and testing methods. The concentration of iron in the new cultivar polished rice was increased by 3 times than that of the original cultivar.