采用我室自己创建的相对恒定微环境小室培养法，对新生大鼠海马进行了原代分离培养。在不用抑制胶质细胞生长的药物情况下，成功地得到了相对单一的神经细胞培养物和单神经元网络，为进一步开展神经生物学研究，提供了很好的体外模型。 Myeloma created a relatively constant micro-environment chamber culture method, the newborn rat hippocampus were isolated and cultured. In the absence of drugs that inhibit glial cell growth, a relatively single network of neural cell cultures and single neurons has been successfully obtained, providing a good in vitro model for further neurobiology studies.