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目的:建立HPLC法同时测定续断配方颗粒中绿原酸、马钱苷、咖啡酸、川续断皂苷Ⅵ的含量。方法:采用Wondasil C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱;流速1.0 m L/min;检测波长212 nm;柱温35℃。结果:绿原酸、马钱苷、咖啡酸、川续断皂苷Ⅵ的进样量分别在0.09~0.9μg(r1=0.9997)、0.049~0.49μg(r_2=0.9997)、0.014~0.14μg(r3=0.9998)、0.484~4.84μg(r4=0.9999)范围内呈良好的线性关系,平均加样回收率为101.16%、97.47%、97.69%、100.52%。结论:该方法准确、可靠、重复性良好,可用于续断配方颗粒的质量控制。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of chlorogenic acid, loganin, caffeic acid, and Dictyosaponin Ⅵ in Semen Granula. Methods: The mobile phase was eluted with Wondasil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.1% phosphoric acid. The flow rate was 1.0 m L / min. The detection wavelength was 212 nm. The column temperature was 35 ℃. Results: The injection volume of chlorogenic acid, loganin, caffeic acid and Chuan Duan saponin Ⅵ were 0.09 ~ 0.9μg (r1 = 0.9997), 0.049 ~ 0.49μg (r_2 = 0.9997), 0.014 ~ 0.14μg = 0.9998) and 0.484 ~ 4.84μg (r4 = 0.9999). The average recoveries were 101.16%, 97.47%, 97.69% and 100.52%, respectively. Conclusion: The method is accurate, reliable and reproducible. It can be used for the quality control of formula particles.