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目的 :观察早期糖尿病大鼠血清对培养视网膜M櫣ｌｌｅｒ细胞 (retinalM櫣ｌｌｅｒｃｅｌｌｓ,RMCs)增殖的影响 .方法 :将链脲霉素 (streptozotocin ,STZ)诱导糖尿病大鼠病程 2wk时的血清 ,以DMEM培养基稀释 (5 0 ,10 0及 2 0 0mL·L-1)后作用于体外培养的大鼠RMCs,采用四唑盐 (tetrazolium ,MTT)还原比色法 ,测定MTT反应的吸光度 (A值 ) .结果 :大鼠血清能促进RMCs增殖 ,并呈现浓度依赖性 ,而早期糖尿病大鼠血清较对照组血清 (正常同龄大鼠的同浓度血清 )有显著的抑制增殖的作用 (P <0 .0 5 ) ,并随着血清浓度的增加 ,抑制率增加 ,5 0 ,10 0及 2 0 0mL·L-1时 ,其A值分别为 0 .2 0 8± 0 .0 2 0vs 0 .2 31± 0 .0 2 0 ,0 .2 6 7± 0 .0 13vs 0 .30 4± 0 .0 14和 0 .32 7± 0 .0 2 1vs 0 .380± 0 .0 2 4 ,其抑制率分别为 10 .0 % ,12 .2 %和 13.9% .结论 :早期糖尿病大鼠血清对RMCs的增殖有显著的抑制作用 .
OBJECTIVE: To observe the effect of sera of early diabetic rats on the proliferation of retinal Müller cells (RMCs) cultured in vitro.METHODS: Serum of diabetic rat induced by streptozotocin (STZ) After medium dilution (50, 100 and 200 mL·L-1), RMCs were cultured in vitro. MTT colorimetry was used to determine the absorbance (A value) of the MTT reaction Results: The serum of rats could promote the proliferation of RMCs in a concentration - dependent manner, while the serum of early diabetic rats had a significant inhibitory effect on proliferation (P <0. 0 5), and with the increase of serum concentration, the inhibitory rate increased at 50, 100 and 200 mL·L-1, the A values were respectively 0.28 ± 0.20 2 vs 0. 2 31 ± 0 .0 2 0, 0.2 6 7 ± 0 .0 13 vs. 0 .30 4 ± 0 .0 14 and 0 .32 7 ± 0 .0 2 1 vs. 0 .80 ± 0 .0 2 4, which inhibited The rates were 10.0%, 12.2% and 13.9% respectively.Conclusion: Serum of early diabetic rats significantly inhibited the proliferation of RMCs.