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目的观察恐惧应激对大鼠血压、心率及一般行为的影响。方法采用条件恐惧应激模型,将64只成年雄性Wistar大鼠随机分为恐惧应激模型组、对照组。通过对大鼠恐惧行为的观察、自发活动试验的测定,动态观察两组大鼠在不同恐惧应激时期行为学方面的变化;通过无创尾动脉测压法,观察不同恐惧应激时程大鼠血压及心率的变化;采用放射免疫学方法测定大鼠在不同恐惧应激时程血清中肌钙蛋白I浓度的变化。结果恐惧组大鼠冻结时间显著高于对照组(t=5.700,P<0.01);恐惧应激组体重在第2周以后增加显著低于对照组(t=2.675,t=2.316;P<0.05;t=3.930,P<0.01);自发活动中恐惧应激组活动次数显著低于对照组(t=2.267,P<0.05;t=2.990,t=3.325;P<0.01;t=2.423,P<0.05);恐惧应激组自发活动中休息时间显著高于对照组(t=2.621,P<0.05;t=3.356,P<0.01;t=2.668,P<0.05;t=2.384,P<0.05);随着应激时程的增加条件恐惧应激组血压显著高于对照组(第2周t=2.887,P<0.05;第3周t=4.263,P<0.01;第4周t=4.414,P<0.01);恐惧模型组心率与对照组相比显著升高(第2周t=2.382,P<0.05;第3周t=1.996,P<0.05;第4周t=1.881,P<0.05);条件恐惧应激模型组大鼠血清cTnI浓度显著高于对照组(第1周t=2.147,P<0.05;第2周t=3.479,P<0.01;第3周t=3.402,P<0.01;第4周t=3.863,P<0.01)。结论条件恐惧应激对大鼠血压、心率、体重、一般活动、血清cTnI都有明显影响。
Objective To observe the effects of fear stress on blood pressure, heart rate and general behavior in rats. Methods Using conditional fear stress model, 64 adult male Wistar rats were randomly divided into fear model group and control group. Through the observation of the fear behavior of rats and the determination of spontaneous activity test, the dynamic changes of behavioral changes of the two groups of rats during different periods of fear and stress were observed dynamically. By means of noninvasive caudal arterial pressure measurement, Blood pressure and heart rate. The radioimmunoassay was used to measure the change of serum troponin I concentration in different phobic stress-induced sera. Results Compared with the control group, the freezing time of the fear group was significantly higher than that of the control group (t = 5.700, P <0.01). The body weight of the fear group increased significantly after 2 weeks (t = 2.675, t = 2.316; ; t = 3.930, P <0.01); The frequency of spontaneous activity of fear-stress group was significantly lower than that of the control group (t = 2.267, P <0.05; t = 2.990, t = 3.325; (P <0.05); t = 2.684, P <0.05; t = 2.668, P <0.05; t = 2.684, P <0.05); The resting time of spontaneous activity in fear and stress group was significantly higher than that in control group (T = 2.887, P <0.05 for the second week; t = 4.263 for the third week, P <0.01; t = 4.414 for the fourth week), and the blood pressure of the fear-stressed group was significantly higher than that of the control group (T = 2.382, P <0.05 in the second week; t = 1.996 in the third week, P <0.05; t = 1.881 in the fourth week, P < (T = 2.147, P <0.05 in the first week; t = 3.479, P <0.01 in the second week; t = 3.402, P = 3.402 in the third week), the level of cTnI in the conditioned fear fear model group was significantly higher than that of the control group <0.01; t = 3.863 in the 4th week, P <0.01). CONCLUSION Conditions Fear stress has a significant effect on blood pressure, heart rate, body weight, general activity and serum cTnI in rats.