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目的:建立用HPLC-ELSD法同时测定红腺忍冬叶抗氧化有效部位中果糖和葡萄糖含量的方法,同时应用此方法研究不同批次红腺忍冬叶抗氧化有效部位果糖和葡萄糖的变化。方法:采用HPLC-ELSD法,对红腺忍冬叶水提液中果糖和葡萄糖的含量测定进行方法学研究,检测12批红腺忍冬叶水提液、30%乙醇沉淀物及其滤液中果糖和葡萄糖的含量。结果:该方法使果糖和葡萄糖得到良好分离。果糖的双对数线性方程为logy=1.2325logx+0.2442,r=0.9999,线性范围为40~2560μg·mL-1,葡萄糖的双对数线性方程为logy=1.2178logx+0.2748,r=0.9995,线性范围为33.75~2160μg·mL-1;果糖和葡萄糖的精密度(RSD)分别为1.45%和1.31%;稳定性分别为2.49%、2.25%;重复性分别为1.72%和1.85%。果糖的平均加样回收率为97.23%,RSD=1.29%,n=9;葡萄糖的平均加样回收率为97.58%,RSD=0.9%,n=9。结论:同一批次红腺忍冬叶,水提液>30%乙醇醇沉滤液>30%乙醇沉淀物。不同批次红腺忍冬叶果糖和葡萄糖含量存在差异,表明果糖和葡萄糖的含量可能与采收时间有关。
OBJECTIVE: To establish a method for the simultaneous determination of fructose and glucose in the antioxidant active fractions of Lonicera lepnea by HPLC-ELSD, and to study the changes of fructose and glucose in the antioxidant active fractions of Lonicera glabra by different methods. Methods: HPLC-ELSD method was used to determine the content of fructose and glucose in the aqueous extract of Lonicera macrophylla. The contents of fructose, Glucose content. Results: This method allows good separation of fructose and glucose. The logarithmic linear equation of fructose was logy = 1.2325logx + 0.2442, r = 0.9999, the linear range was 40 ~ 2560μg · mL-1. The double logarithmic linear equation of glucose was logy = 1.2178logx + 0.2748, r = 0.9995. (RSD) of 1.45% and 1.31%, respectively; the stability was 2.49% and 2.25% respectively; the repeatabilities were 1.72% and 1.85%, respectively. The average recoveries of fructose were 97.23%, RSD = 1.29%, n = 9. The average recoveries of glucose were 97.58%, RSD = 0.9%, n = 9. Conclusion: The same batch of honeysuckle leaf, water extract> 30% ethanol alcohol filtrate> 30% ethanol precipitate. Different batches of Lonicerae heterophylla leaf fructose and glucose content differences, indicating that fructose and glucose content may be related to the harvest time.