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采用表面增强拉曼光谱技术结合快速溶剂前处理方法快速分析芹菜中苯醚甲环唑农药残留。利用乙腈提取芹菜汁,采用N-丙基乙二胺(PSA)、C18和石墨化碳去除芹菜汁中的脂肪酸、蜡质、色素和固醇等荧光物质,分别采集不同浓度苯醚甲环唑标准溶液和以芹菜汁为基质的苯醚甲环唑溶液表面增强拉曼信号。结果表明,1192 cm-1特征峰强度与以芹菜汁为基质的苯醚甲环唑溶液浓度在1~10 mg/L范围内具有良好的线性关系,相关系数为0.9622。以芹菜汁为基质的苯醚甲环唑溶液的最低检测浓度1 mg/L。方法的回收率为92.4%~113.2%,相对标准偏差RSD在8.83%~12.4%之间。在对4个未知浓度的样本进行t检验时,参考值与预测值之间无显著差异。该方法操作简单,单个样本检测时间在20 min内完成,基本满足国家规定的残留限量要求,可为蔬菜中农药残留快速检测装置开发奠定基础。
Rapid Determination of Difenoconazole Pesticide Residues in Celery by Surface Enhanced Raman Spectroscopy and Rapid Solvent Pretreatment. The celery juice was extracted with acetonitrile, and the fluorescent substances such as fatty acids, waxes, pigments and sterols in celery juice were removed by using N-propyl ethylenediamine (PSA), C18 and graphitized carbon. The concentrations of difenoconazole The standard solution and the celery juice-based difenoconazole solution surface enhanced Raman signal. The results showed that the intensity of 1192 cm-1 had a good linear relationship with the concentration of difenoconazole solution in the range of 1 ~ 10 mg / L, the correlation coefficient was 0.9622. Celery juice-based difenoconazole solution of the lowest detection concentration of 1 mg / L. The recoveries of the method ranged from 92.4% to 113.2%, and the relative standard deviations (RSDs) ranged from 8.83% to 12.4%. There was no significant difference between the reference and predicted values when t-tests were performed on four samples of unknown concentration. The method is easy to operate and the detection time of a single sample is completed within 20 min, which basically meets the requirements of residue limits prescribed by the state, which lays the foundation for the rapid development of pesticide residue detection devices in vegetables.