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目的探讨围产期低剂量氯化三丁基锡(TBTCl)暴露对子代雄性小鼠青春期精子质量的影响。方法将20只妊娠6 d的SPF级昆明小鼠随机分为4组,分别为100、10、1μg/kg TBTCl染毒组和对照(玉米油)组,每组5只。从妊娠第6天开始,于每天同一时间经口灌胃染毒,染毒容量为1 ml/kg,至哺乳期结束(出生后21 d)。于出生后28 d开始观察包皮分离的日龄。出生后第49天,取附睾做精子计数,并统计精子畸形情况。结果与对照组比较,100μg/kgTBTCl染毒组小鼠的精子计数显著下降(P<0.01),各剂量TBTCl染毒组小鼠精子畸形率显著升高(P<0.05,P<0.01);且随着TBTCl染毒剂量的升高,小鼠精子计数呈下降趋势,精子畸形率呈升高趋势。而各剂量TBTCl染毒组小鼠包皮分离时间仅略有延迟。结论围产期低剂量TBTCl暴露可导致青春期雄性小鼠精子质量下降。
Objective To investigate the effect of perinatal low dose TBTCl exposure on adolescent sperm quality in offspring male mice. Methods Twenty Kunming mice were randomly divided into 4 groups (n = 10, 10, 1 μg / kg TBTCl) and control (corn oil) groups, with 5 mice in each group. From the 6th day of gestation, oral gavage was performed at the same time every day. The exposure dose was 1 ml / kg until the end of lactation (21 days after birth). The age of prepuce was observed at 28 days after birth. On the 49th day after birth, sperm count was taken from the epididymis and sperm deformity was measured. Results Compared with the control group, the sperm counts of mice in the TBTCl group were significantly decreased (P <0.01), and the sperm deformity rates of the mice in the TBTCl group were significantly increased (P <0.05, P <0.01); and With the increase of TBTCl dose, the sperm count of mice showed a downward trend and the sperm deformity rate showed an increasing trend. While the mice in TBTCl exposure group had only a slight delay in the separation of prepuce. Conclusion Perinatal low dose TBTCl exposure can lead to the decline of sperm in adolescent male mice.