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目的制备人巨细胞病毒（ＨＣＭＶ）ｐ５２蛋白特异性抗原。②方法用ＰＣＲ技术扩增ＨＣＭＶｐ５２蛋白ＩｇＭ抗原决定簇编码区ＤＮＡ片段，克隆到表达载体ｐＢＶ２２０中，转化Ｅ．ｃｏｌｉＤＨ５α株，用氨苄青霉素抗性和质粒酶切图谱分析法筛选阳性克隆，经温控诱导其表达。③结果重组克隆能有效表达天然蛋白ｐ５２，ＥＬＩＳＡ检测重组ｐ５２蛋白具良好的抗原特异性。④结论通过基因工程制备的重组ｐ５２蛋白可作为ＨＣＭＶ血清学诊断的良好抗原。
Objective To prepare human cytomegalovirus (HCMV) p52 protein specific antigen. Methods The DNA fragment coding for the IgM epitope of HCMVp52 protein was amplified by PCR and cloned into expression vector pBV220. The positive clones were screened by ampicillin resistance and plasmid restriction enzyme digestion, and their expression was induced by temperature control. Results The recombinant clones effectively expressed p52 protein, and the recombinant p52 protein detected by ELISA showed good antigen specificity. ④ Conclusion Recombinant p52 protein prepared by genetic engineering can be used as a good antigen for serological diagnosis of HCMV.