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用ＨＴＮ病毒姬鼠型Ｍ基因型特异性引物，异硫氰酸胍一步法提取ＲＮＡ，ｎｅｓｔｅｄＲＴＰＣＲ检测鼠肺和厩真厉螨体内７６１１８株病毒ＲＮＡ，扩增产物经琼脂糖凝胶电泳和点印迹杂交证实。结果表明，姬鼠型７６１１８株病毒液和叮刺感染病毒乳鼠后第１０天厩真厉螨组织悬液接种乳鼠第９天鼠肺、叮剌后第１０天和３０天螨，均检出病毒ＲＮＡ。３０只螨样本提取ＲＮＡ也能扩增出病毒ＲＮＡ。家鼠型ＵＲ株感染样本不能被扩增。该方法具有较高特异性和敏感性，操作简单，结合原位分子杂交，可用于螨媒传播ＨＦＲＳＶ的研究。
HTN virus Kita M genotype-specific primers, guanidine thiocyanate one-step extraction of RNA, nestedRT PCR detection of mouse lung and stable real mites body 76-118 strains of viral RNA, amplified products by agarose gel Confirmation of electrophoresis and dot blot hybridization. The results showed that the agar type 76 118 strains of virus and stings infected with the virus 10 days after feeding neonatal stool Myzus persicae tissue suspension on the 9th day rat pups lungs, 10 days and 30 days after the bite assassination mites, Virus RNA was detected. Extracting RNA from 30 mite samples also amplifies the viral RNA. Home murine UR strain infected samples can not be amplified. The method has high specificity and sensitivity, simple operation, combined with in situ molecular hybridization, which can be used for the study of mite-borne HFRSV.