论文部分内容阅读
目的:探讨发酵支原体(Mycoplasma fermentans,Mf)、梨支原体(Mycoplasma pirum,Mpi)及穿通支原体(Myco-plasma penetrans,Mpe)致死性感染免疫缺陷大鼠血清TNF-α、IL-1β、IL-6和IL-10含量与重要器官损伤之间关系。方法:清洁级SD大鼠61只,分免疫抑制组(47只)、非免疫抑制组(7只)和生理盐水对照组(7只)。免疫抑制组预先经环磷酰胺隔天注射(50 mg/kg.d)3次制备免疫抑制大鼠模型,再分别腹腔注射0.6 ml 3种支原体标准菌株Mf、Mpi、Mpe以及本实验室分离株(W12)和无菌生理盐水;非免疫抑制组和生理盐水对照组取正常大鼠腹腔分别注射相同剂量Mf和生理盐水。取大鼠的肝、肾和脑组织做电镜观察,同时分别取大鼠血液采用ELISA方法检测其肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和白细胞介素10(IL-10)的含量变化。结果:Mf感染组大鼠血清TNF-α、IL-1β和IL-6的含量明显升高(P<0.01);免疫抑制3种支原体感染组血清TNF-α、IL-1β和IL-6的含量均明显低于非免疫抑制Mf感染组(P<0.01),而IL-10含量却显著高于非免疫抑制Mf感染组(P<0.01)。电镜结果显示大鼠的肝、肾和脑组织内细胞间质高度水肿、线粒体肿胀、器官实质变性坏死。结论:发酵支原体、梨支原体以及穿通支原体对免疫缺陷机体产生进一步的细胞免疫抑制作用,更易使免疫缺陷机体发生多器官衰竭甚至致死性感染。
Objective: To investigate the changes of serum TNF-α, IL-1β, IL-6 in Mycoplasma fermentans (Mf), Mycoplasma pirum (Mpi) and lethal Mycoplasma penetrans (Mpe) And the relationship between IL-10 content and vital organ damage. Methods: Sixty clean SD rats were divided into immunosuppressive group (n = 47), nonimmunosuppressive group (n = 7) and saline control group (n = 7). Immunosuppressive groups were immunosuppressed rat models by intraperitoneal injection of cyclophosphamide (50 mg / kg.d) three times in advance and then intraperitoneal injection of 0.6 ml of three kinds of mycoplasma-containing standard strains Mf, Mpi, Mpe and the laboratory isolates (W12) and sterile saline; non-immunosuppressive group and normal saline control group were injected normal mice intraperitoneally with the same dose of Mf and saline. The liver, kidney and brain tissues of rats were observed under electron microscope. At the same time, the levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), interleukin 6 IL-6) and interleukin-10 (IL-10) content changes. Results: The levels of serum TNF-α, IL-1β and IL-6 in Mf infection group were significantly increased (P <0.01). The levels of TNF-α, IL-1β and IL-6 (P <0.01), while the content of IL-10 was significantly higher than that of non-immunosuppressed Mf infection group (P <0.01). Electron microscopy showed that rat liver, kidney and brain tissue interstitial edema, mitochondria swelling, organ degeneration and necrosis. Conclusion: Mycoplasma fermentor, Mycoplasmapilum and Mycoplasma penetrans have further cellular immunosuppressive effects on immune-deficient cells, making it more likely to cause multiple organ failure or even lethal infection in immune-deficient organisms.