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目的:利用NucliSens EasyQ方法进行HIV-1病毒载量检测,探索病毒载量的指导意义,并分析实验质控的关键点。方法:对2008年3月-2010年12月浙江省各地送检的2129例血浆样本进行HIV-1病毒载量检测,对样本信息和实验结果进行统计分析。结果:研究对象的CD4淋巴细胞数(X)与病毒载量(Y)呈负相关,线性方程为lg(Y)=-0.002X+4.397(X<800),R=0.342,P<0.01,N=431。实验结果的总无效率为5.0%(106/2129),无效结果主要是磁性硅胶或硫氰酸胍残留引起(占63.2%),代码4(无扩增)和代码9(弱扩增)最常见共占83.0%(88/106)。结论:病毒载量对病程监控和预测及抗病毒治疗具有重大的指导意义;EasyQ方法实验质控关键点是避免磁性硅胶和硫氰酸胍的残留。
OBJECTIVE: To use the NucliSens EasyQ method to detect HIV-1 viral load and explore the guiding significance of viral load, and to analyze the key points of quality control experiment. Methods: HIV-1 viral load was detected in 2129 plasma samples from March 2008 to December 2010 in Zhejiang Province, and the sample information and experimental results were statistically analyzed. Results: The number of CD4 lymphocytes (X) and the viral load (Y) were negatively correlated with the linear equation lg (Y) = -0.002X + 4.397 (X <800) N = 431. The overall ineffectiveness of the experimental results was 5.0% (106/2129). The ineffective results were mainly caused by magnetic silica gel or guanidine thiocyanate residues (63.2%), code 4 (no amplification) and code 9 Commonly accounted for 83.0% (88/106). Conclusion: The viral load is of great guiding significance for disease course monitoring and prediction and antiviral therapy. The key point of quality control in EasyQ method is to avoid the residues of magnetic silica gel and guanidine thiocyanate.