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目的：探讨内毒素（ＬＰＳ）诱导急性肺损伤（ＡＬＩ）时血小板激活因子（ＰＡＦ）含量变化情况及“神农３３”注射液对ＰＡＦ及ＡＬＩ的影响。方法：给Ｗｉｓｔａｒ大鼠静脉注射ＬＰＳ，制成ＡＬＩ模型；采用反相高效液相色谱法检测ＬＰＳ攻击后１小时大鼠血浆ＰＡＦ含量，观察ＬＰＳ攻击后３小时大鼠肺湿、干重，光镜观察大鼠肺病理形态学变化。结果：ＡＬＩ组动物的肺湿重、湿干重比、肺含水量均明显高于正常对照组（Ｐ均＜０．０５），肺病理组织学改变明显且严重。ＡＬＩ组大鼠血浆ＰＡＦ含量明显高于正常对照组（Ｐ＜０．０１），单独给予“神农３３”注射液无明显降低ＰＡＦ的作用（Ｐ＞０．０５），而以“神农３３”注射液保护的大鼠ＰＡＦ含量明显低于ＡＬＩ组，同时肺损伤也较轻。结论：ＰＡＦ在ＬＰＳ诱导的ＡＬＩ中起重要作用，“神农３３”注射液有拮抗ＰＡＦ及保护肺脏的作用
Objective: To investigate the changes of platelet activating factor (PAF) in acute lung injury (ALI) induced by endotoxin (LPS) and the effect of “Shennong 33” injection on PAF and ALI. Methods: The Wistar rats were injected intravenously with LPS to make ALI model. The content of PAF in rat plasma one hour after LPS challenge was detected by reverse-phase high performance liquid chromatography (RP-HPLC), and the lung wet weight, dry weight and light Microscopic observation of pathological changes of lung in rats. Results: The lung wet weight, wet-dry weight ratio and lung water content in ALI group were significantly higher than those in normal control group (all P <0.05). The histopathology of ALI group changed obviously and severely. Plasma PAF level in ALI group was significantly higher than that in normal control group (P <0.01). Shennong 33 injection alone did not significantly reduce the effect of PAF (P> 0.05) PAF levels in liquid-protected rats were significantly lower than those in the ALI group with less lung injury. Conclusion: PAF plays an important role in LPS-induced ALI. Shennong 33 injection can antagonize PAF and protect the lungs