论文部分内容阅读
分析比较了棉铃虫质型多角体病毒江苏株Ａ、Ｂ两种类型的离体复制特性。ＨａＣＰＶ－Ａ型（ＣＰＶ１４型）病毒可在多种昆虫细胞系中复制，而Ｂ型病毒只能在同源细胞系中增殖；Ａ型病毒的感染率和细胞内游离病毒粒子的滴度均高于Ｂ型病毒；感染细胞持续传代表明，ＨａＣＰＶ－Ａ型病毒感染的ＨＡ－８３１细胞在连续传代７次后，感染率从最初的１０．６％上升到８０％以上，反之，Ｂ型病毒感染的细胞，传代９次后已不能形成典型的多角体。同时，我们还率先成功地建立了ＣＰＶ的空斑测定技术，并初步尝试了混合型ＨａＣＰＶ的分离纯化。
The in vitro replication characteristics of two strains of cotton bollworm strain polyhedrosis virus Jiangsu were analyzed and compared. The HaCPV-A (CPV14) virus replicates in many insect cell lines, whereas the B virus only proliferates in the homologous cell line; both the infection rate of Type A virus and the intracellular free viral particles are high The continuous passage of infected cells showed that the infection rate of HA-831 cells infected with HaCPV-A virus increased from the first 10.6% to over 80% after being passaged seven times in a row. Conversely, Infected cells, after passage 9, can not form a typical polyhedron. At the same time, we also took the lead in successfully establishing the CPV plaque assay technology, and initially tried hybrid HaCPV purification.