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采用ICP MS、凝胶过滤层析及超滤技术 ,通过动物实验研究了长时间、低剂量镧作用后 ,镧在Wistar大鼠肝脏中的代谢累积及其物种分布。结果表明 ,随着给药剂量的增加及作用时间的增长 ,镧在肝脏中的累积量有规律地增大 ;停止给药一段时间后 ,积累在肝脏中的镧有不同程度的代谢 ,其代谢速度很慢 ;累积在肝脏中的镧主要分布于分子量大于 6 0 0 0 0的水溶性蛋白中 ;经SephacrylS 2 0 0层析分离得到的六个蛋白洗脱峰中均含有稀土 ,在第一个洗脱峰中含量最大 ,约占肝脏含镧大分子蛋白 (分子量大于 6 0 0 0 0 )总量的 88%
The effects of lanthanum on the metabolism and distribution of lanthanum in the liver of Wistar rats were studied by means of ICP MS, gel filtration chromatography and ultrafiltration. The results showed that the cumulative amount of lanthanum in the liver increased regularly with the increase of dose and time, and lanthanum accumulated in the liver stopped metabolism after a certain period of time, The lanthanum accumulated in the liver is mainly distributed in the water-soluble protein with a molecular weight greater than 60000. The rare earths contained in the six protein elutions separated by Sephacryl S020 chromatography, The peak elution peak content, accounting for the liver lanthanum macromolecular protein (molecular weight greater than 6 0 0 0) the total amount of 88%