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目的:研究葡萄糖对35%TBSAⅢ°烫伤大鼠肠内补液时小肠电解质溶液吸收率的影响。方法:雄性Wistar大鼠30只,随机分为:假烫补林格氏液组(假烫LR组,n=10);烫伤补林格氏液组(烫伤LR组,n=10)和烫伤补葡萄糖-林格氏液组(烫伤LR/G组,林格氏液中加入20g/L的葡萄糖粉,n=10)。烫伤组采用沸水法(100℃,15 s)造成35%TBSAⅢ°烫伤。各组于烫伤后0.5h按1/2派克兰公式量和速率(2mL/kg.1%TBSA-1)进行肠内补液。测定烫伤后4h内肠道对水分和Na+的吸收率,以及伤后4h肠黏膜血流量和Na+-K+-ATP酶活性。结果:烫伤LR组小肠对水和Na+的吸收率明显下降(P<0.05);两烫伤组之间比较,烫伤LR/G组对水和Na+的吸收优于烫伤LR组,在伤后2h有显著差别(P<0.05);伤后4h烫伤LR/G组肠黏膜血流量和Na+-K+-ATP酶活性也显著高于烫伤LR组(P<0.05)。结论:35%TBSAⅢ°烫伤大鼠肠内补液时,小肠对葡萄糖电解质溶液的吸收率明显下降;葡萄糖可以提高烫伤休克肠内复苏时小肠的吸收率,并且与提高肠黏膜血流量和Na+-K+-ATP酶活性有关。
Objective: To study the effect of glucose on the absorption rate of small intestine electrolyte solution in 35% TBSA Ⅲ scald rats during enteral rehydration. Methods: Thirty male Wistar rats were randomly divided into five groups: sham-burn group (sham LR group, n = 10) Glucose-Ringer’s solution (scalded LR / G, Ringer’s solution added 20g / L glucose powder, n = 10). Scald group using boiling water method (100 ℃, 15 s) caused by 35% TBSA Ⅲ ° scald. Each group was subjected to enteral rehydration at a rate of 1/2 Paxlain Formula (2 mL / kg.1% TBSA-1) 0.5 h after scald. The intestinal absorption of water and Na + within 4h after scalding were measured, and the intestinal mucosal blood flow and Na + -K + -ATPase activity were also measured 4h after injury. Results: The absorptivity of water and Na + in LR group decreased significantly (P <0.05). Compared with LR group, LR and G group had better absorption of water and Na + than scalded LR group (P <0.05). Intestinal mucosal blood flow and Na + -K + -ATPase activity in LR / G group were also significantly higher than those in scalded LR group 4h after scald (P <0.05). CONCLUSION: When the intestinal fluid rehydration in 35% TBSA Ⅲ scald rats, the intestine’s absorption rate of glucose electrolyte solution decreased obviously. Glucose can improve the intestinal absorption rate of intestine after scald shock and improve the intestinal mucosal blood flow and Na + -K + -ATP enzyme activity.