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目的：研究全反维甲酸（ＡＴＲＡ）等干扰因子对肺癌细胞株Ａ５４９侵袭、增殖及ＩＣＡＭ－１分子表达的调节作用。方法：根据细胞穿过铺有Ｍａｔｒｉｇｅｌ胶多孔膜数量检测细胞侵袭力，用改良龙胆紫吸收比色计数法检测细胞增殖，以流式细胞分析和酶联免疫吸附试验检测ＩＣＡＭ－１、ｓＩＣＡＭ－１表达水平。结果：Ａ５４９ 经ＡＴＲＡ处理后，细胞表面ＩＣＡＭ－１表达降低，体外侵袭力明显降低，体外增殖明显抑制；ＩＦＮ α不增加细胞ＩＣＡＭ－１表达，但抑制体外增殖及体外侵袭力；ＩＬ－１ 明显增强细胞表面ＩＣＡＭ－１表达及培养上清ｓＩＣＡＭ－１含量，体外侵袭力有增高趋势。结论：ＡＴＲＡ、ＩＦＮ α、ＩＬ－１ 对Ａ５４９ 细胞体外增殖及ＩＣＡＭ－１、ｓＩＣＡＭ－１表达有不同的调节作用，而对其转移潜能具有不同调变作用。
Objective: To study the regulation effect of all-anti-retinoic acid (ATRA) and other interfering factors on the invasion and proliferation of lung cancer cell line A549 and the expression of ICAM-1 molecule. METHODS: The cell invasiveness was measured by the number of cells coated with porous membranes of Matrigel, cell proliferation was measured by modified gentian violet absorption colorimetric counting method, and ICAM-1 and sICAM- were measured by flow cytometric analysis and enzyme-linked immunosorbent assay. 1 Expression level. RESULTS: After treated with ATRA, the expression of ICAM-1 on the surface of A549 cells was decreased, the invasiveness was significantly reduced in vitro, and the proliferation was inhibited in vitro. IFN α did not increase the expression of ICAM-1, but inhibited the in vitro proliferation and in vitro invasiveness; IL-1 significantly The expression of ICAM-1 on the cell surface and the content of sICAM-1 in the culture supernatant were increased, and the invasiveness in vitro was increased. Conclusion: ATRA, IFN α and IL-1 have different effects on the proliferation of A549 cells and the expression of ICAM-1 and sICAM-1 in vitro, but have different modulation effects on their metastatic potential.