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苯中毒动物骨髓或白细胞DNA含量和合成速率的变化已有报道,但结果不很一致,且仅见用染色测定法;而关于环核苷酸水平的变化则未见报道。为探讨血细胞减少和造血功能抑制是否与苯对核酸代谢的影响有关,我们对苯中毒大鼠白细胞和骨髓的DNA含量以及骨髓有核细胞的CAMP、cGMP水平进行了初步研究。材料与方法(一)将成年雌性大鼠随机分成苯中毒组与正常对照组,每组5～7只。苯中毒组包括静式吸入染毒和灌胃两种途径,前者苯浓度为14.6mg/l,每天3小时,染毒期共12天至半年,后者剂量为1000mg/kg,以
Benzene poisoning animal bone marrow or leukocyte DNA content and the rate of change has been reported, but the results are not very consistent, and only see the staining assay; and on the cyclic nucleotide changes have not been reported. To investigate whether cytopenia and inhibition of hematopoietic function are related to the effects of benzene on nucleic acid metabolism, we investigated the DNA content of leukocytes and bone marrow of benign rats and the levels of CAMP and cGMP in bone marrow nucleated cells. Materials and methods (a) Adult female rats were randomly divided into benzene poisoning group and normal control group, each group of 5 to 7. Benzene poisoning group, including inhalation and intravenous injection of static and gavage of the two ways, the former benzene concentration of 14.6mg / l, 3 hours a day, the exposure period of 12 days to six months, the latter dose of 1000mg / kg, with