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目的探讨Raf激酶抑制蛋白(Raf kinase inhibitor protein,RKIP)在肺鳞癌和肺腺癌组织中的表达及其与侵袭转移的关系。方法应用RT-PCR和Western blot方法检测50例肺鳞癌、44肺腺癌组织及对应癌旁正常肺组织中RKIP的表达。结果在肺鳞癌和肺腺癌中,RKIP mRNA及蛋白的表达明显低于癌旁正常肺组织(P<0.05),RKIP的表达与肺癌的分化程度、TNM分期、淋巴结转移、生存期有关(P<0.05),与患者的性别、年龄、吸烟史、肿瘤的大小及组织学类型无关(P>0.05)。结论RKIP的表达减弱或缺失在肺癌的发生及侵袭转移过程中发挥重要作用,可作为肺癌的一项预后指标。
Objective To investigate the expression of Raf kinase inhibitor protein (RK kinase) in lung squamous cell carcinoma and lung adenocarcinoma and its relationship with invasion and metastasis. Methods RT-PCR and Western blot were used to detect the expression of RKIP in 50 cases of squamous cell carcinoma, 44 cases of lung adenocarcinoma and corresponding normal lung tissues. Results The expression of RKIP mRNA and protein in lung squamous cell carcinoma and lung adenocarcinoma was significantly lower than that in adjacent normal lung tissues (P <0.05). The expression of RKIP was correlated with the differentiation of lung cancer, TNM stage, lymph node metastasis and survival P <0.05), but not with the gender, age, smoking history, tumor size and histological type (P> 0.05). Conclusions The weakening or deletion of RKIP expression plays an important role in the occurrence and invasion of lung cancer and may be used as a prognostic indicator of lung cancer.