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目的：研究成纤维细胞介导的Ｇ－ＣＳＦ基因治疗对于大剂量化疗后结肠癌小鼠造血功能的影响。方法：分别将重组人Ｇ－ＣＳＦ（ｒｈＧ－ＣＳＦ）及分泌Ｇ－ＣＳＦ的成纤维细胞埋植于接受大剂量化疗后的结肠癌小鼠腹腔内，观察结肠癌小鼠造血功能的改变。结果：ｒｈＧ－ＣＳＦ注射疗法及Ｇ－ＣＳＦ基因疗法不但均可明显提高结肠癌小鼠外周血白细胞数量（Ｐ＜０．０５），还能明显减轻大剂量化疗后荷瘤小鼠外周血白细胞的降低程度；Ｇ－ＣＳＦ基因治疗后，大剂量化疗后结肠癌小鼠外周血血小板数量降低程度也轻于对照小鼠，但其恢复过程比较缓慢；并能明显提高化疗及未经化疗的结肠癌小鼠的脾脏质量（Ｐ＜０．０１），促进化疗后小鼠脾脏和骨髓ＣＦＵ－ＧＭ，ＣＦＵ－ＭＫ，ＣＦＵ－Ｓ水平的恢复。结论：Ｇ－ＣＳＦ基因治疗较ｒｈＧ－ＣＳＦ注射疗法更显著地减轻大剂量化疗后外周血白细胞的降低程度、促进骨髓和脾脏造血功能的恢复、提高化疗小鼠外周血白细胞和血小板的数量
Objective: To study the effect of fibroblast-mediated G-CSF gene therapy on hematopoietic function of colon cancer mice after high-dose chemotherapy. METHODS: Recombinant human G-CSF (rhG-CSF) and G-CSF-secreting fibroblasts were implanted in the abdominal cavity of colon cancer mice receiving high-dose chemotherapy, and the hematopoietic function of colon cancer mice was observed. RESULTS: Both rhG-CSF injection and G-CSF gene therapy not only significantly increased the number of white blood cells in colon cancer mice (P<0.05), but also significantly reduced the peripheral blood leukocytes in tumor-bearing mice after high-dose chemotherapy. The degree of reduction; after G-CSF gene therapy, the decrease in platelet count in peripheral blood of mice with colon cancer after high-dose chemotherapy was also milder than in control mice, but the recovery process was slow; and it could significantly increase chemotherapy and chemotherapy-free colon cancer. The spleen mass of mice (P<0.01) promoted the recovery of spleen and bone marrow CFU-GM, CFU-MK and CFU-S levels after chemotherapy. Conclusion: G-CSF gene therapy can significantly reduce the decrease of peripheral blood leukocytes after high-dose chemotherapy, promote the recovery of bone marrow and spleen hematopoietic function, and increase the number of peripheral blood leukocytes and platelets in chemotherapy mice compared with rhG-CSF injection therapy.