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目的 :建立中药材三七叶中人参皂苷Rb3 的含量测定方法。方法 :HPLC法 ,流动相 :乙腈 水 (30∶70 ) ,检测波长 2 0 5nm。结果 :人参皂苷Rb3 含量测定线性范围为 1.36～ 8.19μg ,平均回收率10 1.2 % ,RSD为 1.7%。结论 :方法可靠 ,简单可行 ,为建立三七叶的质量标准提供了科学依据。
Objective : To establish a method for the determination of ginsenoside Rb3 in Chinese medicinal materials Sanqi leaves. Method : HPLC, mobile phase : acetonitrile water (30∶70), detection wavelength of 20 nm. Results: The linear range of ginsenoside Rb3 content was 1.36～ 8.19μg, the average recovery rate was 10 1.2 %, RSD was 1.7%. Conclusion: The method is reliable, simple and feasible, and provides scientific basis for establishing the quality standard of Panax notoginseng leaves.