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目的观察愈疡灵软膏对糖尿病溃疡大鼠创面愈合的影响及其可能作用机制。方法 60只Wistar大鼠随机分为对照组、模型组、凡士林组、愈疡灵组,每组15只。除对照组外其余各组采用高脂高糖饲料联合小剂量链脲佐菌素液腹腔注射法制备糖尿病大鼠模型,后各组大鼠均制作溃疡模型。于溃疡造模后第2天愈疡灵组大鼠创面以愈疡灵软膏涂敷其上,约厚2 mm,外用无菌纱布包扎固定;凡士林组采用凡士林油纱外敷;对照组及模型组采用生理盐水纱布外敷,各组每日换药1次。观察给药第3、7、14天各组大鼠创面愈合情况,并采用HE染色法检测创面组织形态结构的变化,检测溃疡组织中Wnt信号通路相关的β-连环素(β-catenin)、糖原合成激酶-3β(GSK-3β)、R-脊椎蛋白3(Rspo-3)蛋白及mRNA的变化。结果与对照组比较,模型组、凡士林组、愈疡灵组大鼠在第3、7、14天创面愈合率,创面组织中β-catenin、Rspo-3蛋白及mRNA表达均降低,GSK-3β蛋白及mRNA表达均升高(P<0.05)。与模型组、凡士林组比较,愈疡灵组大鼠的创面愈合率、创面组织病理学变化、创面组织中β-catenin、GSK-3β、Rspo-3蛋白及mRNA的表达水平均得到不同程度改善,尤其以第14天最为明显(P<0.05)。结论愈疡灵软膏可促进糖尿病溃疡大鼠创面的愈合,其机制可能通过对Wnt/β-catenin信号通路进行调节。
Objective To observe the effect of Yuyinling Ointment on wound healing in diabetic ulcer rats and its possible mechanism. Methods Sixty Wistar rats were randomly divided into control group, model group, Vaseline group and Yuyangling group, 15 rats in each group. Except the control group, diabetic rats model was made by intraperitoneal injection of high-fat and high-sugar diet combined with low-dose streptozotocin solution, and ulcer model was made in each group. On the 2nd day after ulcer modeling, the wounds of Yuanyingling group were coated with Yuyangling ointment, about 2 mm thick, and externally fixed with sterile gauze. The Vaseline group was coated with Vaseline gauze. The control group and model group Saline topical application, each group dressing 1 day. The wound healing of the rats in each group was observed on the 3rd, 7th and 14th day after administration. The morphological changes of wounds were observed by HE staining. The β-catenin, Changes of Glycogen Synthase Kinase-3β (GSK-3β), R-vertebrate 3 (Rspo-3) Protein and mRNA. Results Compared with the control group, the wound healing rate, the expression of β-catenin and Rspo-3 protein and mRNA in the wounds of the model group, Vaseline group and Yuanyingling group were decreased on the 3rd, 7th and 14th days. GSK-3β The protein and mRNA expression increased (P <0.05). Compared with the model group and the Vaseline group, the wound healing rate, wound histopathological changes, the expression levels of β-catenin, GSK-3β, Rspo-3 protein and mRNA in the wounds of Yujianling group were all improved to some extent , Especially on the 14th day (P <0.05). Conclusion Yuyangling Ointment can promote wound healing in diabetic ulcer rats, and its mechanism may be through the regulation of Wnt / β-catenin signaling pathway.