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通过升高色谱柱温度,结合线性梯度洗脱的分离模式调整分离选择性,发展了一种氨基酸的2,4-二硝基氟苯柱前衍生化高效液相色谱分析方法,也将该方法用于大黄中氨基酸含量的测定。所发展的方法采用Kromasil C18,(250mm×4.6mm I.D.5μm)色谱柱,44℃柱温,以3段线性梯度可以在30分钟内基线分离17种基本氨基酸,方法具有普适性好、稳定性高等特点。
A method for the determination of 2,4-dinitrofluorobenzene by precolumn derivatization of amino acids was developed by increasing the temperature of the column and adjusting the separation selectivity in combination with the separation mode of linear gradient elution. This method For the determination of amino acids in rhubarb. The developed method uses Kromasil C18 column (250mm × 4.6mm ID5μm) and column temperature of 44 ℃ to separate 17 kinds of basic amino acids within 30 minutes by a 3-step linear gradient. The method has good universality and stability Higher characteristics.