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中耳胆脂瘤造成骨质破坏的确切机理还不很清楚,有各种理论解释。本文作者认为白细胞介素1(IL—1)在其中起重要作用。为证实其理论,作者采用以下实验:①病人手术取出的胆脂瘤标本作石蜡切片,免疫过氧化物酶染色,证明在胆脂瘤的活性骨质破坏区有高浓度的IL—1,尤为明显的是IL—1主要在单核细胞及与胆脂瘤相连接的骨细胞内。②用新生小鼠头骨制备成骨细胞,加上不同浓度的IL—1,放免法测定IL—1刺激成骨细胞产生前列腺素E_2的量及胶原酶活性。结果当IL—1浓度为100pg/ml时前列腺素E_2产量最大。IL—1的浓度在10～1 000p/ml不刺激成骨细胞产生胶原酶。③从小鼠的
The exact mechanism of bone destruction caused by middle ear cholesteatoma is not well understood and there are various theoretical explanations. The authors believe interleukin-1 (IL-1) plays an important role in this. To confirm its theory, the authors used the following experiments: ① patients with cholesteatoma specimens removed for paraffin sections, immunoperoxidase staining, in the presence of active bone destruction area of cholesteatoma high concentration of IL-1, especially It is clear that IL-1 is mainly found in monocytes and in osteocytes that are associated with cholesteatoma. (2) Osteoblasts were prepared from newborn mouse skull, and different concentrations of IL-1 were added to determine the amount of prostaglandin E2 and collagenase activity of IL-1-stimulated osteoblasts by radioimmunoassay. Results The production of prostaglandin E_2 was the highest when IL-1 concentration was 100pg / ml. The concentration of IL-1 at 10 ~ 1000p / ml does not stimulate osteoblasts to produce collagenase. ③ from the mouse