目的评价二盐酸组胺和组胺Ｈ２受体拮抗剂西咪替丁对人胃粘膜上皮细胞ＧＥＳ１增殖和迁移的影响．方法细胞增殖检测采用酸性磷酸酶细胞原位计数法．细胞迁移用细胞损伤检测法．同时，用Ｆｌｕｏ３ＡＭ为荧光标记物在粘附式细胞仪上进行了细胞内游离钙测定．结果西咪替丁浓度为１０５，１０６，１０７，１０８和１０９ｍｏｌ／Ｌ时，细胞数分别比对照组增加５８２％、６００％、７３７％和７４５％（Ｐ＜００１），细胞迁移数（１０６ｍｏｌ／Ｌ）为对照组的５９２％（Ｐ＜００５）；而组胺对细胞的增殖和迁移无明显影响．西咪替丁刺激可使ＧＥＳ１细胞内（Ｃａ２＋）ｉ浓度呈双向改变．结论西咪替丁可促进体外人胃粘膜上皮细胞的增殖和迁移，这可能是Ｈ２受体拮抗剂抗溃疡的机制之一，其途径可能部分是通过钙离子信号系统介导的． Objective To evaluate the effects of cimetidine dihydrochloride and histamine H2 receptor antagonist cimetidine on the proliferation and migration of human gastric mucosal epithelial cells GES 1. Methods Cell proliferation assay using acid phosphatase in situ cell counting. Cell damage detection method for cell migration. At the same time, Fluo  3  AM fluorescence markers in the adherent cytometer intracellular free calcium determination. Results When the concentration of cimetidine was 105, 106, 107, 108 and 109 mol / L, the number of cells increased 582%, 600% and 737 respectively compared with the control group % And 745% respectively (P <001). The number of cell migration (10  6mol / L) was 592% of the control group (P <005), while the histamine had no effect on cell proliferation and migration Clearly affected. Cimetidine stimulation can make GES  1 intracellular (Ca2 +) i concentration was bi-directional changes. Conclusion Cimetidine can promote the proliferation and migration of human gastric mucosal epithelial cells in vitro, which may be one of the mechanisms of H2 receptor antagonist anti-ulcer. The pathway may be partly mediated by calcium signaling system.