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[目的]探讨PARP-1抑制剂PJ34干预治疗对大鼠局灶脑缺血/再灌注损伤的影响。[方法]用线栓法建立局灶脑缺血/再灌注模型(Ⅰ组),分别经左侧侧脑室注射PJ34(Ⅱ组)、生理盐水(Ⅲ组)和假手术组(Ⅳ组);通过Klenow标记及TUNEL染色技术检测鼠脑中单、双链DNA断裂,分别用原位杂交及免疫组化技术检测鼠脑中PARP-1蛋白的表达,同时测定脑组织及血浆中TNF–α、IL-6及IL-8含量变化和细胞凋亡情况。[结果]与Ⅰ组比较,Ⅲ组鼠脑缺血侧DNA单、双链断裂,PARP-1的表达均无明显差异,Ⅱ组缺血侧脑中Klenow及TUNEL阳性细胞数均明显减少,PARP-1的表达明显减少,TNF-α、IL-6及IL-8含量和凋亡减少。[结论]PARP-1抑制剂PJ34对脑缺血/再灌注损伤具有保护作用。
[Objective] To investigate the effect of PARP-1 inhibitor PJ34 on focal cerebral ischemia / reperfusion injury in rats. [Methods] Focal cerebral ischemia / reperfusion model was established by thread occlusion (group Ⅰ). PJ34 (group Ⅱ), normal saline (group Ⅲ) and sham operation group (group Ⅳ) were injected into the left lateral ventricle. Single and double stranded DNA was detected by Klenow labeling and TUNEL staining. The expression of PARP-1 protein in rat brain was detected by in situ hybridization and immunohistochemistry respectively. The levels of TNF-α, IL-6 and IL-8 content changes and apoptosis. [Results] Compared with group Ⅰ, there was no significant difference of DNA single strand and double strand break, PARP-1 expression in ischemia group Ⅱ in group Ⅲ, and the number of Klenow and TUNEL positive cells in group Ⅱ was significantly decreased -1 expression was significantly reduced, TNF-α, IL-6 and IL-8 content and apoptosis decreased. [Conclusion] PARP-1 inhibitor PJ34 has a protective effect on cerebral ischemia / reperfusion injury.