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目的:探讨荧光分析中四种常用溶剂水、乙醇、氯仿、四氯化碳主要拉曼散射光的变化规律及对稀溶液荧光分析的影响。方法:采用荧光分光光度计分别检测四种常用溶剂在不同激发光照射下主要的拉曼散射光。结果:在不同波长激发光照射下,可检测到溶剂水有频移为0.348μm-1和0.172μm-1的拉曼散射峰,乙醇有频移为0.306、0.150μm-1和0.075μm-1的拉曼散射峰,氯仿的拉曼散射峰频移为0.307μm-1和0.155μm-1。四氯化碳的拉曼散射强度很弱,且与瑞利散射部分重叠,未能得到其频移值。结论:由频移的定义,推导出拉曼峰的波长与激发光波长及频移的关系:λRaman=1-Δλσex.λex。由此公式可以预测拉曼散射峰的位置,了解其对激发光谱和荧光光谱的影响,为荧光分析中测定波长的选择起到重要的指导作用。
OBJECTIVE: To investigate the regularity of Raman scattering of four common solvents for water, ethanol, chloroform and carbon tetrachloride in fluorescence analysis and its effect on the fluorescence analysis of dilute solution. Methods: Fluorescence spectrophotometer was used to detect the main Raman scattered light of four commonly used solvents under different excitation lights. Results: The Raman scattering peaks of 0.348μm-1 and 0.172μm-1 in water of solvent were detected under different wavelengths of excitation light. The frequency shift of ethanol was 0.306, 0.150μm-1 and 0.075μm-1 The Raman scattering peaks of chloroform were 0.307μm-1 and 0.155μm-1, respectively. The intensity of Raman scattering of carbon tetrachloride is very weak and partially overlaps with Rayleigh scattering, failing to obtain its frequency shift value. Conclusion: From the definition of frequency shift, the relationship between the wavelength of Raman peak and the wavelength and frequency shift of excitation light is derived: λ Raman = 1-Δλσex.λex. The formula can predict the position of the Raman scattering peak, understand its impact on the excitation spectrum and the fluorescence spectrum, and play an important guiding role in the determination of the wavelength of the fluorescence analysis.