论文部分内容阅读
本文报告肿瘤坏死因子（ＴＮＦ）和ｒ－干扰素（ＩＦＮ－ｒ）诱导小鼠胰岛细胞ＭＨＣ－ＤＲ抗原表达对胰岛素分泌的影响。实验结果表明：（１）当ＴＮＦ浓度为５０～１００Ｕ／ｍｌ时未见胰岛细胞表达ＭＨＣ－ＤＲ抗原，而５０Ｕ／ｍｌｒ－ＩＦＮ可诱导少量胰岛细胞（８．５±２．９％）表达ＭＨＣ－ＤＲ抗原；（２）１００Ｕ／ｍｌＴＮＦ和１００Ｕ／ｍｌｒ－ＩＦＮ合并使用，ＭＨＣ－ＤＲ抗原阳性细胞明显增多（８０．２５±４．１１％）；（３）两种因子单独或合并作用均能使体外培养的胰岛细胞的胰岛素分泌量增加（Ｐ＜０．０１）。上述结果提示，胰岛细胞ＭＨＣ－ＤＲ抗原的诱导表达本身并不影响其分泌胰岛素的功能。
This article reports the effects of tumor necrosis factor (TNF) and r-interferon (IFN-r) on MHC-DR antigen expression in mouse pancreatic islet cells on insulin secretion. The results showed that: (1) no islet cells expressed MHC-DR antigen when the concentration of TNF was 50-100U / ml, but a small amount of islet cells (8.5 ± 2.9%) were induced by 50U / (2) 100U / ml TNF and 100U / ml r-IFN combined, MHC-DR antigen positive cells increased significantly (80.25 ± 4.11%); (3) The two factors alone or in combination can Insulin secretion increased in cultured islet cells (P <0.01). The above results suggest that the induction of MHC-DR antigen expression in pancreatic islet itself does not affect its insulin secretion function.