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目的 :探讨康莱特 (KLT)对肾癌细胞系 (GRC 1)的放射增敏作用和机制。材料和方法 :利用细胞克隆技术进行剂量 -存活曲线分析 ,末端脱氧核苷酰转移酶法检测细胞凋亡 ,免疫细胞化学法分析bcl-2和PCNA基因表达。结果 :0 2mg/mlKLT组D0 值为 90 44cGy ,空白对照组和空白乳组D0 值分别为 13 1 0 9cGy和 13 9 40cGy ,统计学处理P <0 0 5。 0 2mg/mlKLT处理GRC 1细胞后 ,M 1区阳性细胞为 89 76 % ,空白乳组为1 0 2 %。 0 2mg/mlKLT组GRC 1细胞bcl 2和PCNA基因表达的标记指数 (LI)分别为 6 6 0 %和 15 2 % ,空白乳组LI分别为 2 5 %和 0 % ,统计学处理差异显著 (P <0 0 1)。结论 :0 2mg/mlKLT具有明显提高GRC 1细胞放射敏感性的作用 ,其作用机制是诱发GRC 1细胞凋亡 ,抑制GRC 1细胞bcl 2基因表达和上调PCNA基因表达
Objective: To investigate the radiosensitization and mechanism of KLT on renal cell carcinoma (GRC 1). Materials and Methods: Cell-clone technique was used for dose-survival curve analysis. Terminal deoxynucleotidyl transferase assay was used to detect apoptosis. Immunocytochemistry was used to analyze bcl-2 and PCNA gene expression. RESULTS: The D0 value of the 0 2mg/ml KLT group was 90 44cGy, and the D0 values of the blank control group and the blank milk group were 13109cGy and 13 9 40cGy, respectively. Statistical analysis was P 0 05. After treatment of GRC 1 cells with 0 2 mg/ml KLT, the positive cells in the M 1 region were 89.76 % and the blank milk group was 102 %. The labeling index (LI) of the expression of bcl 2 and PCNA genes in GRC 1 cells of 0 2 mg/ml KLT group was 66.0 % and 1 2 %, respectively, and the blank milk group LI was 2 5 % and 0 % respectively. Statistically significant differences were observed (P < 0.05). P < 0 0 1). Conclusion : 0 2 mg/ml KLT can significantly increase the radiosensitivity of GRC 1 cells. Its mechanism is to induce apoptosis of GRC 1 cells, inhibit the expression of bcl 2 gene and upregulate PCNA gene expression in GRC 1 cells.