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[目的]研究厚竹叶片叶绿素荧光的异质性。[方法]运用调制荧光成像系统Mini-IMAGING-PAM的叶绿素荧光和成像技术,测量厚竹叶片叶绿素荧光,计算各荧光参数。[结果]PSII最大量子产量Fv/Fm和叶片吸光系数Abs均质性较高,实际量子产量Y、非光化学淬灭NPQ/4、光化学淬灭qP和相对电子传递速率PS/50的异质性都很强。Y、qP和PS/50的变化趋势基本相同,横向呈现两边高、中间低的趋势,即靠近叶片边缘数值高,靠近叶脉部分数值低。叶片纵向(从上到下)的Y、qP和PS/50变异系数逐渐减小,说明从叶子尖端到叶子基部异质性逐渐降低。PS/50和NPQ/4呈相反变化趋势。[结论]厚竹叶片各荧光参数有不同的异质性。
[Objective] The research aimed to study the heterogeneity of chlorophyll fluorescence in thick bamboo leaves. [Method] The chlorophyll fluorescence of bamboo leaves was measured by using the fluorescence imaging system Mini-IMAGING-PAM and the imaging technique. The fluorescence parameters were calculated. [Result] The heterogeneity of Fv / Fm of maximum quantum yield of PSII and Abs of Abscisic acid absorption coefficient Abs were high, and the actual quantum yield Y, the non-photochemical quenching NPQ / 4, the photochemical quenching qP and the relative electron transfer rate PS / 50 Very strong. The trends of Y, qP and PS / 50 are basically the same, with the trend of high and low in both sides in horizontal direction, that is, the value near the leaf edge is high and the value near the leaf vein is low. The coefficients of variation of Y, qP and PS / 50 in the leaf longitudinal direction (from top to bottom) gradually decreased, indicating that the heterogeneity gradually decreased from the leaf tip to the leaf base. PS / 50 and NPQ / 4 showed the opposite trend. [Conclusion] The fluorescence parameters of bamboo have different heterogeneity.