论文部分内容阅读
用免疫组化ＰＡＰ法观察人体皮肤生前枪弹创标本４０例、死后３０例损伤局部５－ＨＴ的变化，发现生前枪弹创局部５－ＨＴ主要分布于创缘、皮肤乳头层、皮下疏松结缔组织及皮肤附件周围结缔组织内，皮肤网状层结缔组织内５－ＨＴ分布较少；死后８分钟内形成的枪弹创改变与生前相同。免疫组化ＰＡＰ法亦证实人体皮肤肥大细胞颗粒内不含５－ＨＴ，表明用免疫组化ＰＡＰ法观察损伤局部５－ＨＴ的变化对生前死后损伤的鉴别有重要价值，并为前人生化方法测定结果提供了形态学依据。甲苯胺蓝染色，发现生前枪弹刨距创缘０～４．０ｍｍ区域内肥大细胞脱颗粒率均＞５０％，与死后枪弹创标本比较有显著差异，故可认为肥大细胞脱颗粒率变化可为生前死后损伤鉴别依据之一。
Using immunohistochemical PAP method observed 40 cases of human skin bullet wound specimens, 30 cases of post-mortem local 5-HT damage changes and found that prenatal bullet wound local 5-HT are mainly located in wound edge, skin papillary layer, subcutaneous loose connective tissue And the skin around the connective tissue connective tissue, skin reticular connective tissue less 5-HT distribution; within 8 minutes after the death of bullets formed the same change as before. Immunohistochemical PAP method also confirmed that human skin mast cell particles do not contain 5-HT, indicating that the use of immunohistochemical PAP method to observe changes in the local injury 5-HT damage to the identification of prenatal injury is of great value, and for the previous biochemical The results of the method provide morphological evidence. Toluidine blue staining and found that the size of mast cells degranulation rate was> 50% in the range of 0 ~ 4.0mm, which was significantly different from that of post-mortem bullet wounds. Therefore, it is considered that the degranulation rate of mast cells may be changed One of the evidences for the identification of post-mortem injuries.