论文部分内容阅读
目的观察慢性轻度不可预见性应激对支气管哮喘大鼠哮喘病情的影响。方法通过对哮喘组及实验组大鼠腹腔注射卵蛋白,并雾化吸入卵蛋白制备支气管哮喘模型。在雾化吸入卵蛋白后对实验组进行连续28 d的慢性轻度不可预见性应激。此后以无创小动物呼吸功能测量仪检测大鼠呼吸功能、HE染色观察肺组织病变、并支气管灌洗液中细胞数量及分类。结果①与正常组比较,哮喘组呼吸功能降低(P<0.05);与哮喘组比较,哮喘组大鼠呼吸功能进一步降低(P<0.05)。②与正常组比较,哮喘组大鼠支气管灌洗液中白细胞总数及嗜酸性粒细胞均显著升高(P<0.05);与哮喘组比较,哮喘组大鼠支气管灌洗液中白细胞总数及嗜酸性粒细胞均显著升高(P<0.05)。③与哮喘组大鼠比较,实验组大鼠支气管壁上皮细胞较哮喘组排列更加紊乱,部分结构破坏,白细胞浸润较哮喘组更加显著,肺泡失去正常形态。结论慢性轻度不可预见性应激可以通过加重哮喘病情,临床工作中应注重对哮喘患者进行心理疏导、避免心理应激。
Objective To observe the effects of chronic mild unpredictable stress on asthma in asthmatic rats. Methods Bronchial asthma model was established by intraperitoneal injection of ovalbumin in asthmatic rats and experimental rats, and inhalation of ovalbumin. The experimental group was treated for 28 consecutive days with mild mild unpredictable stress after nebulized ovalbumin. After that, the respiratory function of rats was measured by noninvasive small animal respiratory function measuring instrument. The pathological changes of lung tissue were observed by HE staining, and the number and classification of cells in bronchoalveolar lavage fluid were also observed. Results ① Compared with the normal group, the respiratory function of the asthma group was decreased (P <0.05). Compared with the asthma group, the respiratory function of the asthma group was further decreased (P <0.05). ② Compared with the normal group, the total number of leukocytes and eosinophils in the bronchial lavage fluid of the asthma group were significantly increased (P <0.05). Compared with the asthma group, the total number of leukocytes and the number of leukocytes in the bronchial lavage fluid of the asthma group Acidic granulocytes were significantly increased (P <0.05). Compared with the asthma group, the bronchial wall epithelial cells in the experimental group were more disordered than those in the asthma group, some of the structures were destroyed, and the leukocyte infiltration was more significant than that in the asthma group. The alveoli lost their normal morphology. Conclusion Chronic mild unpredictable stress can aggravate the condition of asthma. In clinical practice, psychological stress should be avoided in patients with asthma.