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[目的]研究去乙酰化转移酶抑制剂TSA对人肝癌HepG2细胞的作用及其FHIT表达的影响。[方法]培养的人肝癌HepG2细胞随机分为两组:对照组给予等量DMSO,实验组给予终浓度分别为125、250、500、1 000、2 000nmol/L的TSA,培养24 h后收集细胞,MTT比色法检测细胞活性,TUNEL法检测细胞凋亡率,逆转录聚合酶链反应(RT-PCR)和免疫细胞化学检测FHIT的mRNA和蛋白表达水平。[结果]与对照组相比,经TSA处理的细胞增殖速度明显减慢,TUNEL阳性细胞百分率随TSA浓度的升高呈剂量依赖性增高(P﹤0.01),细胞FHIT mRNA表达增强(P﹤0.01),FHIT蛋白表达差异具有统计学意义(P﹤0.01)。[结论]TSA可能通过抑制HDACs的活性,上调FHIT表达,诱导细胞凋亡而抑制肝癌细胞生长。
[Objective] To investigate the effect of deacetylase inhibitor TSA on human hepatoma HepG2 cells and its effect on FHIT expression. [Methods] HepG2 human hepatocellular carcinoma cells were randomly divided into two groups: the control group was given the same amount of DMSO, the experimental group were given TSA at the final concentration of 125, 250, 500, 1 000, 2 000 nmol / L, The cell viability was measured by MTT assay. The apoptosis rate was detected by TUNEL assay. The mRNA and protein expression of FHIT were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry. [Results] Compared with the control group, the proliferation rate of TSA-treated cells was significantly slowed down. The percentage of TUNEL-positive cells increased with the increase of TSA in a dose-dependent manner (P <0.01) and the expression of FHIT mRNA increased ), FHIT protein expression difference was statistically significant (P <0.01). [Conclusion] TSA may inhibit the growth of hepatoma cells by inhibiting the activity of HDACs, up-regulating FHIT expression and inducing apoptosis.