我们使用纯化的ＨＳＰ２７、ＨＳＰ６０和ＨＳＰ７０，采用Ｗｅｓｔｅｒｎｂｌｏｔ方法，检测５３名飞行员血浆中相应的抗体水平，并探讨了他们可能的意义。结果发现，飞行员血浆ＨＳＰｓ抗体检出率，ＨＳＰ２７为１５．１％，ＨＳＰ６０为２０．７％，ＨＳＰ７０为２９．４％，同时检测出两种抗体的百分率为１８．９％。这些提示了飞行员可能曾经常遭受过热等多种不良环境应激和高热、低氧等不良生理应激，其可能的机制为这些因素诱导ＨＳＰｓ的产生、细胞死亡和免疫功能混乱所致。 Using purified HSP27, HSP60 and HSP70, we detected the corresponding antibody levels in plasma of 53 pilots using Western blotting and explored their possible implications. The results showed that the detection rate of plasma HSPs in pilots was 15.1% for HSP27, 20.7% for HSP60, 29.4% for HSP70 and 18.9% for both antibodies. These suggest that pilots may have suffered from many adverse environmental stresses such as overheating and other unhealthy physiological stresses such as hyperthermia and hypoxia. Their possible mechanisms are the induction of HSPs, cell death and immune disorders by these factors.