报道一种微量测定抗结核药物异烟肼的新方法，方法是基于异烟肼对鲁米诺－锰（Ⅱ）－高碘酸钾化学发光反应的增敏作用，建立了微量异烟肼的流动注射化学发光分析法。本法检测限（ＤＬ）是３ × １０８ｇ／ｍＬ；线性浓度范围为１．０×１０７～１．０×１０５ｇ／ｍＬ；对１．０×１０６ｇ／ｍＬ异烟肼的测定（ｎ＝６），相对标准偏差小于２％。 A new method for the determination of isoniazid as an antimicrobial agent was reported. The method was based on the sensitization of isoniazid to luminol-manganese (Ⅱ) -potassium periodate chemiluminescence reaction and established the method of isoniazid Flow Injection Chemiluminescence Analysis. The detection limit (DL) for this method was 3 × 108 g / mL; the linear range was 1.0 × 107 to 1.0 × 105 g / mL; the determination of isoniazid 1.0 × 106 g / mL (n = 6) , Relative standard deviation less than 2%.