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目的:培养无排卵功血子宫内膜上皮细胞(EEC),以使其可作为无排卵功血的体外实验模型之一。方法:在取材、培养条件和细胞纯化等方面对EEC原代培养的方法作了改进。结果:培养成活率:无排卵型功血EEC与正常增殖期EEC相比无差异;单纯性增生过长EEC高于增生期样EEC。结论:.通过改进培养方法,克服了污染、纯化及细胞数少等问题,成功分离培养了无排卵型功血EEC,为研究无排卵功血提供了一个重要的实验工具。
Objective: To culture anovulatory endometrial epithelial cells (EEC) so that it can be used as an in vitro experimental model of anovulatory dysfunctional uterine bleeding. Methods: The method of primary culture of EEC was improved in terms of material, culture conditions and cell purification. Results: The survival rate of culture: There was no difference in EEC between anovulatory dysfunctional uterine bleeding and normal proliferative phase EEC; the hyperplasia of simple hyperplasia EEC was higher than that of proliferative phase EEC. Conclusion: By improving the culture method, it overcomes the problems of pollution, purification and small number of cells, successfully isolated and cultured anovulatory dysfunctional uterine bleeding EEC, which provides an important experimental tool for the study of anovulatory dysfunctional uterine bleeding.