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目的探讨免疫印迹试验在丙肝确认检测中的应用。方法将135份HCV初筛为阳性的血清标本再进行抗-HCV的ELISA检测,仍为阳性的再用荧光PCR法检测HCV-RNA以及特异性更高的补充确认试验,即免疫印迹试验(WB)。结果初检为阳性的135份血清ELISA检测后仍然为阳性,HCV-RNA的检测结果为<1.0×10~3IU/ml有28份,>1.0×10~3IU/ml的有107份。WB结果为阳性115份,阴性19份,不确定1份。结论多种原因可能会导致ELISA法检测丙肝抗体时出现假阳性,而核酸检测灵敏度、病毒血症消退或病毒基因变异等多种因素的影响可能也会出现抗-HCV结果阳性而RNA检测结果为阴性的情况。因此建议对于抗-HCV初检阳性的标本进一步用免疫印迹试验进行复检,以排除假阳性结果。
Objective To investigate the application of western blot in hepatitis C confirmation test. Methods A total of 135 serological samples of HCV positive for screening were further tested by anti-HCV ELISA and still positive. The detection of HCV-RNA by fluorescence PCR and the more specific confirmatory confirmation test, ie, Western blotting (WB) ). Results The results of the first seizure test showed that 135 serum samples were still positive after ELISA test. The results of HCV-RNA assay showed that there were 28 samples with <1.0 × 10 ~ 3IU / ml and 107 samples with> 1.0 × 10 ~ 3IU / ml. WB results for the positive 115, negative 19, not sure 1. Conclusion A variety of reasons may lead to the false positive of ELISA for detecting hepatitis C antibody. The influence of many factors such as sensitivity of detection of nucleic acid, regression of viremia or viral gene variation may lead to positive anti-HCV results and RNA test result is Negative situation. Therefore, it is recommended that the specimens positive for anti-HCV test be further re-examined by Western blotting to exclude false-positive results.