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人血浆高密度脂蛋白(HDLs)的主要亚组分为HDL_2(密度1.063～1.125)及HDL_3(密度1.125～1.210),据认为它们是从不同代谢途径生成的。HDL_3的前体是由肝或小肠所分泌,而HDL_2的前体是由含甘油三酯的脂蛋白在降解过程中所产生的。作者采用高效性液体层析法测定了肝硬化患者及正常对照者的血清HDL_2和HDL_3胆固醇水平。将Ⅰ型(G5000PW)和Ⅱ型(G3000SW)液凝胶渗透型柱联合,并与反应型高效色谱仪相连接(HLC805型),将受检血样置于液柱,用酶法测出20μl血清
The major sub-components of human plasma HDLs are HDL_2 (density 1.063-1.125) and HDL_3 (density 1.125-1.210), which are thought to be produced from different metabolic pathways. The precursors of HDL_3 are secreted by the liver or small intestine, while the precursor of HDL_2 is produced during the degradation by triglyceride-containing lipoproteins. The authors used high performance liquid chromatography to determine serum HDL 2 and HDL 3 cholesterol levels in patients with cirrhosis and controls. Type I (G5000PW) and type II (G3000SW) liquid gel permeation column were combined and connected to a reaction-type high performance chromatograph (HLC805 type). The test blood sample was placed on a liquid column, and 20 μl of serum