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目的探讨转录因子FOXO3a在PTEN调节RAD51表达中的作用。方法利用Western印迹技术分析PTEN缺陷与否时,总FOXO3a以及细胞核内FOXO3a磷酸化的情况;转染外源AKT到PTEN野生型细胞或外源PTEN和失去激酶活性的AKT(AKT-DN)到PTEN缺陷型细胞,Western印迹检测核内FOXO3a的磷酸化情况。结果 PTEN缺失导致细胞核内FOXO3a磷酸化水平增高;外源PTEN可以降低PTEN缺陷型细胞核内FOXO3a的磷酸化水平,外源AKT可以增加PTEN野生型细胞核内FOXO3a的磷酸化水平;沉默FOXO3a在一定程度上导致RAD51表达水平下降。结论 FOXO3a可以结合到RAD51基因的启动子区,PI3K/AKT/FOXO3a信号通路是PTEN调节RAD51表达的重要方式之一。
Objective To investigate the role of transcription factor FOXO3a in the regulation of RAD51 expression by PTEN. Methods Western blotting was used to analyze the phosphorylation of FOXO3a and FOXO3a in the nucleus after PTEN deficiency or not. Transfection of exogenous AKT to PTEN wild-type cells or exogenous PTEN and AKT-DN lost kinase to PTEN Defective cells, Western blotting to detect phosphorylation of FOXO3a in the nucleus. Results The deletion of PTEN led to the increase of FOXO3a phosphorylation in the nucleus. Exogenous PTEN decreased the phosphorylation of FOXO3a in PTEN deficient nuclei, and the exogenous AKT increased the phosphorylation of FOXO3a in wild-type PTEN cells. The silencing of FOXO3a to a certain extent Resulting in decreased RAD51 expression. Conclusion FOXO3a can bind to the promoter region of RAD51 gene. PI3K / AKT / FOXO3a signaling pathway is one of the important ways PTEN regulates the expression of RAD51.