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Aim:To characterize the coexpression of survivin,an inhibitor of apoptosis(IAF),and human telomerase reversetranscriptase(hTERT)in human testes with varying spermatogenic function.Methods:Transcript levels of survivinmRNA and hTERT mRNA were determined in normal testes(n=11)and testes with defective spermatogenesis(n=28)using real-time reverse-transcription polymerase chain reaction(RT-PCR).The histological work-up wasperformed according to a modified Johnsen score.Results:Expressions of both survivin and hTERT were highest atmedian levels of 96.8 and 709 in normal spermatogenesis and dropped to 53.3 and 534 in testes with postmeioticspermatogenic arrest(n=10).In severe spermatogenic failure(n=18),survivin expression was lacking in mostspecimens(n=16),whereas at least low levels of testicular hTERT expression were largely detectable with a normal-ized expression of 73 in premeiotic spermatogenic arrest(n=7)and 45 in patients with Sertoli cell-only syndrome(SCOS)(n=3).Both survivin and hTERT expressions increased with a progressing Johnsen score(P for trend =0.001).Conclusion:Although both survivin and hTERT are correlated with spermatogenic function,they show differentexpression patterns in testes of infertile patients.These findings substantiate results from studies in the rodent testissuggesting a predominant expression of survivin in meiotically dividing germ cells.(Asian J Androl 2006 Jan;8:95-100)
Aim: To characterize the coexpression of survivin, an inhibitor of apoptosis (IAF), and human telomerase reversetranscriptase (hTERT) in human testes with varying spermatogenic function. Methods: Transcript levels of survivin mRNA and hTERT mRNA were determined in normal testes (n = 11 ) and testes with defective spermatogenesis (n = 28) using real-time reverse-transcription polymerase chain reaction (RT-PCR) .The histological work-up wasperformed according to a modified Johnsen score. Results: Expressions of both survivin and hTERT were highest at severe levels of 96.8 and 709 in normal spermatogenesis and dropped to 53.3 and 534 in testes with postmeiotics pematogenic arrest (n = 10) .In severe spermatogenic failure (n = 18), survivin expression was lacking in mostspecimens (n = 16) least low levels of testicular hTERT expression were largely detectable with a normal-ized expression of 73 in premeiotic spermatogenic arrest (n = 7) and 45 in patients with Sertoli cell-only syndrome (SCOS) (n = 3) hTERT expressions increased with a progressing Johnsen score (P for trend = 0.001). Conclusions: Both both survivin and hTERT are correlated with spermatogenic function, they show differentexpression patterns in testes of infertile patients. The findings substantiate results from studies in the rodent testissuggesting a predominant expression of survivin in meiotically dividing germ cells. (Asian J Androl 2006 Jan; 8: 95-100)