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目的观察硫化氢对高糖诱导的血管内皮细胞炎症反应的影响并探讨其机制。方法将人脐静脉内皮细胞(HUVECs)分为4组:A组为正常糖浓度组(5.5 mmol/L),B组为高糖组(16.7 mmol/L),C组为高糖(16.7 mmol/L)+硫氢化钠(100μmol/L)组,D组为正常糖浓度(5.5 mmol/L)+硫氢化钠(100μmol/L)组,处理24 h后,采用RT-PCR、Western Blot和电泳迁移率实验(EMSA)检测核因子-κB(NF-κB)的表达和活性;处理48 h后,检测环氧合酶-2(COX-2)的表达。结果①与A组相比,B组COX-2蛋白表达量明显增加(P<0.01),COX-2 mRNA表达量明显增加(P<0.01);与B组相比,C组COX-2蛋白表达量明显降低(P<0.05),COX-2mRNA表达量明显降低(P<0.01)。②与A组相比,B组细胞质内NF-κB蛋白表达量明显降低(P<0.05);与B组相比,C组细胞质内NF-κB蛋白表达量明显增加(P<0.01);与A组相比,B、C、D组NF-κB mRNA表达差异无统计学意义(P>0.05)。③与A组相比,B组NF-κB活性增加;与B组相比,C组NF-κB活性降低。D组各指标差异无统计学意义(P>0.05)。结论硫化氢可以抑制高糖诱导的内皮细胞的炎症反应,其机制可能与抑制COX-2的表达有关。
Objective To observe the effect of hydrogen sulfide on inflammation induced by high glucose in vascular endothelial cells and to explore its mechanism. Methods Human umbilical vein endothelial cells (HUVECs) were divided into four groups: normal glucose concentration group (5.5 mmol / L), high glucose group (16.7 mmol / L) and high glucose group (L) + sodium hydrosulfide (100μmol / L) group, D group was normal glucose concentration (5.5mmol / L) + sodium hydrosulfide (100μmol / L) Electrophoretic mobility shift assay (EMSA) was used to detect the expression and activity of nuclear factor-κB (NF-κB), and the expression of cyclooxygenase-2 (COX-2) was detected 48 h after treatment. Results Compared with group A, the expression of COX-2 protein in group B was significantly increased (P <0.01) and the expression of COX-2 mRNA was significantly increased in group B (P <0.01). Compared with group B, COX- The expression of COX-2 mRNA was significantly decreased (P <0.01). ② Compared with group A, the expression of NF-κB in cytoplasm of group B was significantly decreased (P <0.05); Compared with group B, the expression of NF-κB in cytoplasm of group C was significantly increased (P <0.01); There was no significant difference in NF-κB mRNA expression in group B, C, D between group A and group A (P> 0.05). ③ Compared with group A, NF-κB activity increased in group B; Compared with group B, NF-κB activity in group C was decreased. There was no significant difference between each index in group D (P> 0.05). Conclusion Hydrogen sulfide can inhibit the inflammation induced by high glucose in endothelial cells, which may be related to the inhibition of COX-2 expression.