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目的：应用放射免疫分析方法检测急性吗啡耐受过程中大鼠脑室灌流液、中脑导水管周围灰质（ＰＡＧ）及杏仁核中孤啡肽（ＯＦＱ）免疫活性（ｉｒ）的动态变化。结果：（１）每２小时皮下注射盐酸吗啡１次（７ｍｇ／ｋｇ），连续注射８次，造成急性吗啡耐受。此组大鼠脑室灌流液中ＯＦＱｉｒ较生理盐水对照组升高４０％，差异显著（Ｐ＜０．０５）。（２）皮下注射吗啡１、６、８次的大鼠ＰＡＧ中ＯＦＱｉｒ含量呈逐渐增长趋势，分别较生理盐水组升高９％（Ｐ＞０．０５）、３５％（Ｐ＜０．０５）和６０％（Ｐ＜０．０１）。（３）皮下注射１次吗啡的大鼠杏仁核中ＯＦＱｉｒ与生理盐水组相比，降低１１％，但无统计学意义；至第８次吗啡注射后较对照组升高４１％（Ｐ＜０．０５）。结论：多次注射吗啡能引起大鼠脑内ＯＦＱ含量和释放增多，结合以往关于ＯＦＱ在脑内对抗吗啡镇痛的事实表明内源性ＯＦＱ可能参与急性吗啡耐受的形成。
AIM: To detect the dynamic changes of immunocompetence (ir) in rat brain perfusion fluid, periaqueductal gray (PAG) and OFQ in acute morphine tolerance by radioimmunoassay. RESULTS: (1) Morphine hydrochloride was injected subcutaneously once every 2 hours (7 mg / kg) for 8 consecutive injections, resulting in acute morphine tolerance. OFQ ir in the intraventricular perfusate increased by 40% compared with the saline control group, the difference was significant (P <0.05). (2) The contents of OFQir in PAG of rats injected with morphine 1,6,6 times subcutaneously increased gradually by 9% (P> 0.05) and 35% (P <0), respectively. 05) and 60% (P <0.01). (3) OFQir in rat amygdala treated with morphine decreased by 11% compared with saline group, but there was no statistical significance. By the 8th injection of morphine, it was 41% higher than that of control group (P <0.05). Conclusions: Multiple injections of morphine can cause an increase in OFQ content and release in the rat brain. The fact that OFQ acts against morphine analgesia in the brain in the past indicates that endogenous OFQ may be involved in the formation of acute morphine tolerance.