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[目的]观察七氟醚麻醉后对脑血流量不足的老年大鼠认知功能的影响,为临床用药作参考。[方法]24只老年Wistar大鼠随机分为4组,每组6只。分别为对照组(Ⅰ组),2%的七氟醚组(Ⅱ组),3%的七氟醚组(Ⅲ组)和七氟醚+缺血组(Ⅳ组)。各组动物持续分别吸入0%、2%、3%、2%的七氟醚和2Lmin-1O2+6 Lmin-1N2,每天吸入100min,连续吸5d且第Ⅳ组于吸入混合气前1周结扎左侧颈总动脉。用Morris水迷宫测试大鼠的认知功能,每天4次,连续测定5d。水迷宫测试结束后立即处死大鼠,用逆转录-聚合酶链反应方法测定海马NR、NR1和NR2B的mR-NA表达。[结果]①重复测量方差分析结果表明从1d到5d,Ⅰ～Ⅳ组动物的潜伏期和游泳距离有逐日缩短的趋势。到d5各组动物的潜伏期和游泳距离与d1比较差别有统计学意义(P﹤0.05)。②完全随机方差分析结果表明4组NR2B亚单位mRNA表达差别有统计学意义(F=7.79,P﹤0.001)。[结论]七氟醚对老年大鼠术后认知功能呈剂量依赖性抑制作用;脑血流不足影响七氟醚对老年大鼠的认知功能。第Ⅳ组NR2B的mRNA呈现高表达,可能与早前的脑血流不足有关。
[Objective] To observe the effect of sevoflurane anesthesia on the cognitive function of senile rats with cerebral blood flow insufficiency for reference for clinical use. [Methods] Twenty-four aged Wistar rats were randomly divided into 4 groups with 6 rats in each group. They were control group (group Ⅰ), 2% sevoflurane group (group Ⅱ), 3% sevoflurane group (group Ⅲ) and sevoflurane + ischemia group (group Ⅳ). The animals in each group continued to inhale 0%, 2%, 3%, 2% of sevoflurane and 2Lmin-1O2 + 6 Lmin-1N2 for 100 min every day for 5 consecutive days and group IV ligation one week before inhalation Left common carotid artery. Rats were tested for cognitive function using the Morris water maze four times a day for five consecutive days. Immediately after water maze test, rats were sacrificed and mR-NA expression of NR, NR1 and NR2B in the hippocampus was determined by reverse transcription-polymerase chain reaction. [Results] ①Results of repeated measures ANOVA showed that from 1d to 5d, the latency and swimming distance of Ⅰ ~ Ⅳ group animals tended to decrease day by day. There was a significant difference in latency and swimming distance between d5 and d1 animals (P <0.05). ② The results of complete random analysis of variance showed that there was a significant difference in mRNA expression of NR2B subunit among the four groups (F = 7.79, P <0.001). [Conclusion] Sevoflurane can inhibit the cognitive function of senile rats in a dose-dependent manner. The cerebral blood flow insufficiency affects the cognitive function of sevoflurane in senile rats. Group IV NR2B mRNA showed high expression, may be related to the earlier lack of cerebral blood flow.